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Launch the Excel application on your computer and open the workbook where you want to perform subtraction.
Click on the cell where you want the result of the subtraction to appear.
Type the formula for subtraction. For example, to subtract cell A2 from cell A1, type **=A1-A2** in the selected cell.
After typing the formula, press the **Enter** key on your keyboard. The result of the subtraction will be displayed in the selected cell.
Review the displayed result to ensure it is correct. If needed, adjust the formula or cells referenced and repeat the process.